Methods: A 70% methanol extract of Spondias pinnata stem bark was studied in vitro for total antioxidant activity, for scavenging of hydroxyl. BACKGROUND: Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring. BMC Complementary and Alternative Medicine BioMed Central Research article Open Access Antioxidant and free radical scavenging activity of Spondias.
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The extract showed total antioxidant activity with a trolox equivalent antioxidant concentration TEAC value of 0.
Antioxidant and free radical scavenging activity of Spondias pinnata. | PubFacts
Potential of plant method: The percentage inhibition of absorbance was calculated and plotted as a function of the concentration of standard and sample to determine the trolox equivalent antioxidant concentration TEAC.
Recently, many natural antioxidants have been isolated from different plant materials [ 2829 ]. Antioxidantism-free radical and biological defense. Briefly, the plant extract 0.
Hydroxyl radical scavenging assay. The reaction of NO with superoxide. The IC 50 values for scavenging of free radicals were After incubation for 5 min at standard method  with a slight modification.
Extraction The stem bark of S. The mechanism of action of flavonoids is through scavenging or chelation [ 45 ].
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Briefly, the The superoxide radicals generated from dissolved oxygen plant extract 0. Article in Biochem Pharmacol M Fontana et al. Quercetin was used as positive control. The reaction of NO with superoxide. Catalase inactivation is inhibited in the presence of Authors’ contributions the extract, signifying its HOCl scavenging activity.
Superoxide radical scavenging This activity was measured by the reduction of NBT according to a previously antioxirant method [ 11 ]. A crude extract of S. Addition of the plant extract to this pre-formed radical cation reduced it to ABTS in a concentration-dependent manner.
The reductive abilities of Spondias the standard BHT and found to be superior. Antibacterial activity of different plant extracts.
An aqueous extract of this The stem bark of S. Ethnomedicinal plant resources Gallic acid and curcumin were obtained from MP Biomedicals, France.
Antioxidant and free radical scavenging activity of Spondias pinnata – Dimensions
Flavonoids are scavengers of superox- curcumin. The scavenging activity of sample was compared with that of lipoic acid, used as a reference compound. Superoxide and singlet oxygen in milk lipid peroxidation. FEBS Lett S-allylmercaptocysteine scavenges hydroxyl radical and singlet oxygen in vitro and attenuates gentamicininduced oxidative and nitrosative stress and renal damage in vivo.
The supernatants collected from the two phases were mixed in a round bottom flask and concentrated under reduced pressure in a rotary evaporator. All tests were the reaction mixture. This activity was determined according to a previously described method [ 13 ] with minor changes. For the peroxynitrite, singlet oxygen and hypochlorous acid scavenging activities the IC50 values were The plant extract mg yielded The absorbance of the ABTS.
Kurz Family — Anacardiaceae from Spectrochem Pvt. The IC50 value of the that of the reference compound.
Hazra is grateful to the Indian Council of Medical Research, Govern- phosphomolybdic-phosphotungstic acid reagents. Biological oxidants and antioxidants: The phenolic content was evaluated Table 1 of the plant extract and quercetin on superoxide from a gallic acid standard curve. Kasetsart J Nat constants for reaction of hydroxyl radicals. Competing interests The authors declare that they have no competing interests. The pellet was mixed again with ml methanol-water and the entire extraction process was repeated.
The IC 50 value of the standard is 1. All data are expressed as mean oxide inhibition. The total flavonoid content was determined with aluminium chloride AlCl 3 according to a known method [ 24 ] using quercetin as a standard.